4UX3
cohesin Smc3-HD:Scc1-N complex from yeast
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 73.151, 94.801, 284.768 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.300 |
R-factor | 0.27663 |
Rwork | 0.274 |
R-free | 0.32861 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xex |
RMSD bond length | 0.011 |
RMSD bond angle | 1.778 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.480 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.110 | 1.500 |
Number of reflections | 15350 | |
<I/σ(I)> | 17.7 | 2.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 25.6 | 26.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |