4UW9

The crystal structural of archaeal beta-phosphoglucomutase from hyper-thermophilic Pyrococcus sp. Strain ST 04

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	95
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	I 41
Unit cell lengths	94.628, 94.628, 83.124
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.225 - 2.300
R-factor	0.1898
Rwork	0.184
R-free	0.23850
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.013
RMSD bond angle	1.408
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	31.220
High resolution limit [Å]	2.300
Rmerge	0.070
Number of reflections	16290
<I/σ(I)>	43.67
Completeness [%]	99.0
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1