4UW8
Structure of the carboxy-terminal domain of the bacteriophage T5 L- shaped tail fiber with its intra-molecular chaperone domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-08 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.850, 99.290, 286.230 |
| Unit cell angles | 90.00, 91.51, 90.00 |
Refinement procedure
| Resolution | 95.380 - 2.520 |
| R-factor | 0.18497 |
| Rwork | 0.185 |
| R-free | 0.21311 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uw7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.397 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.400 | 2.650 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.140 | 0.430 |
| Number of reflections | 150772 | |
| <I/σ(I)> | 6.3 | 3.5 |
| Completeness [%] | 98.8 | 97.2 |
| Redundancy | 4.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 10 MM TRIS-HCL, 0.1 M SODIUM CITRATE PH 4.0, 12.5 % (W/V) PEG 2000 |
