4UW7
Structure of the carboxy-terminal domain of the bacteriophage T5 L- shaped tail fiber without its intra-molecular chaperone domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 227.430, 58.180, 69.910 |
| Unit cell angles | 90.00, 98.81, 90.00 |
Refinement procedure
| Resolution | 56.190 - 2.520 |
| R-factor | 0.21704 |
| Rwork | 0.213 |
| R-free | 0.26607 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.402 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.200 | 2.590 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.100 | 0.640 |
| Number of reflections | 30585 | |
| <I/σ(I)> | 11.4 | 2.3 |
| Completeness [%] | 96.8 | 95.8 |
| Redundancy | 6.3 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS-HCL PH 8.5, 17.5% (W/V) PEG 4000 |
