4UW2
Crystal structure of Csm1 in T.onnurineus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-07-10 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 57.894, 158.292, 299.275 |
| Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
| Resolution | 30.657 - 2.632 |
| R-factor | 0.2061 |
| Rwork | 0.203 |
| R-free | 0.26090 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.608 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.680 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Rmerge | 0.070 | 0.070 |
| Number of reflections | 78224 | |
| <I/σ(I)> | 40.84 | 4.67 |
| Completeness [%] | 98.2 | 98.5 |
| Redundancy | 6.8 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.2 M AMMONIUM CITRATE PH 7.0, 20% PEG3350 |
