4UV6
Crystal structure of apical membrane antigen 1 from Plasmodium knowlesi
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-05 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 90.320, 105.700, 104.750 |
Unit cell angles | 90.00, 98.04, 90.00 |
Refinement procedure
Resolution | 47.090 - 2.450 |
R-factor | 0.172 |
Rwork | 0.171 |
R-free | 0.22660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1w81 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.150 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.090 | 2.580 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.100 | 0.500 |
Number of reflections | 34848 | |
<I/σ(I)> | 9.7 | 1.9 |
Completeness [%] | 97.3 | 93.3 |
Redundancy | 2.9 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.4 | 0.86M SODIUM CITRATE, 0.1M HEPES PH 8.4 |