4UV3
Structure of the curli transport lipoprotein CsgG in its membrane- bound conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-31 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.934, 372.847, 161.972 |
| Unit cell angles | 90.00, 92.90, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.590 |
| R-factor | 0.29921 |
| Rwork | 0.297 |
| R-free | 0.34903 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uv2 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.364 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.680 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.160 | 0.910 |
| Number of reflections | 102130 | |
| <I/σ(I)> | 6.8 | 1.89 |
| Completeness [%] | 91.6 | 27.3 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 100 MM TRIS-HCL PH 8.0, 8% PEG 4000, 100 MM NACL AND 500 MM MGCL2 |
