4UUX
Competence or damage-inducible protein CinA from Thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-13 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.390, 94.030, 132.380 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.930 - 1.990 |
R-factor | 0.17741 |
Rwork | 0.176 |
R-free | 0.21254 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ct8 |
RMSD bond length | 0.021 |
RMSD bond angle | 1.972 |
Data reduction software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.000 | 2.040 |
High resolution limit [Å] | 1.990 | 1.990 |
Rmerge | 0.060 | 0.560 |
Number of reflections | 62924 | |
<I/σ(I)> | 17 | 2.5 |
Completeness [%] | 99.3 | 99 |
Redundancy | 4.2 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 0.2 M NA2SO4, 0.1 M BIS-TRIS PROPANE (PH 6.5) AND 20% (WT/VOL) PEG 3350 |