4UUW
Competence or damage-inducible protein CinA from Thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-13 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.280, 93.710, 132.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.940 - 1.980 |
R-factor | 0.18217 |
Rwork | 0.180 |
R-free | 0.22436 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ct8 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.866 |
Data reduction software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.000 | 2.030 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.050 | 0.460 |
Number of reflections | 63973 | |
<I/σ(I)> | 12.4 | 2.3 |
Completeness [%] | 99.5 | 99.6 |
Redundancy | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 0.2 M NA2SO4, 0.1 M BIS-TRIS PROPANE (PH 6.5) AND 20% (WT/VOL) PEG 3350 |