4UUR
Cold-adapted truncated hemoglobin from the Antarctic marine bacterium Pseudoalteromonas haloplanktis TAC125
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-15 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.910, 72.250, 88.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.150 - 2.210 |
| R-factor | 0.1856 |
| Rwork | 0.183 |
| R-free | 0.24617 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xyk |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.752 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CaspR |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.250 | 2.330 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.100 | 0.450 |
| Number of reflections | 14322 | |
| <I/σ(I)> | 8.7 | 3.1 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 4.4 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 8% PEG 8000 AND 0.1 M TRIS-HCL (PH 8.5) |
