4UUM
Apo trichomonas vaginalis lactate dehydrogenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-17 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 82.570, 59.610, 86.490 |
Unit cell angles | 90.00, 116.18, 90.00 |
Refinement procedure
Resolution | 44.653 - 1.368 |
R-factor | 0.1286 |
Rwork | 0.128 |
R-free | 0.14020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | HOMOLOGY MODEL BASED ON PDB ENTRY 5MDH |
RMSD bond length | 0.008 |
RMSD bond angle | 1.177 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.700 | 1.400 |
High resolution limit [Å] | 1.400 | 1.370 |
Rmerge | 0.040 | |
Number of reflections | 145693 | |
<I/σ(I)> | 20.8 | 1.42 |
Completeness [%] | 91.5 | 48.9 |
Redundancy | 6.66 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.6 | 44 MG/ML PROTEIN, 100 MM HEPES PH 7.6, 8.3% PEG-6000, 5% MPD |