4UUL
Apo trichomonas vaginalis lactate dehydrogenase L91R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.570, 59.610, 86.490 |
| Unit cell angles | 90.00, 116.18, 90.00 |
Refinement procedure
| Resolution | 30.712 - 1.280 |
| R-factor | 0.1306 |
| Rwork | 0.129 |
| R-free | 0.15450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HOMOLOGY MODEL BASED ON PDB ENTRY 5MDH |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.474 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.700 | 1.310 |
| High resolution limit [Å] | 1.300 | 1.280 |
| Rmerge | 0.050 | 0.820 |
| Number of reflections | 175089 | |
| <I/σ(I)> | 28.2 | 2.61 |
| Completeness [%] | 90.3 | 47.5 |
| Redundancy | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 21 MG/ML PROTEIN, 100 MM HEPES PH 7.0, 10% PEG-6000, 5% MPD |
