4UUJ
POTASSIUM CHANNEL KCSA-FAB WITH TETRAHEXYLAMMONIUM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-19 |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 |
| Unit cell lengths | 154.615, 154.615, 75.953 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.640 - 2.400 |
| R-factor | 0.19436 |
| Rwork | 0.191 |
| R-free | 0.24823 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k4c |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.697 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.050 | 0.580 |
| Number of reflections | 32854 | |
| <I/σ(I)> | 17.1 | 2.1 |
| Completeness [%] | 98.1 | 97.7 |
| Redundancy | 3.31 | 3.31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.4 | CRYSTALLIZATION CONDITIONS: 18-25% PEG400, 50 MM SODIUM REMARK 280 ACETATE, 50 MM MAGNESIUM ACETATE, pH 5.4 |
