4UU4
Crystal structure of LptH, the LptA homologous periplasmic component of the conserved lipopolysaccharide transport device from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 45.000, 45.000, 146.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.020 - 2.751 |
R-factor | 0.2377 |
Rwork | 0.235 |
R-free | 0.29590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2r19 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.911 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.900 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.140 | 0.560 |
Number of reflections | 4383 | |
<I/σ(I)> | 13.2 | 4 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.8 | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 20% 1,4-BUTANEDIOL, 0.1 M HEPES (PH 7.5), 0.2 M NACL. |