4UTS
Room temperature crystal structure of the fast switching M159T mutant of fluorescent protein Dronpa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2013-07-05 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.655, 111.140, 117.572 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.630 - 2.030 |
R-factor | 0.17368 |
Rwork | 0.172 |
R-free | 0.21326 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2z1o |
RMSD bond length | 0.019 |
RMSD bond angle | 2.170 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.820 | 2.150 |
High resolution limit [Å] | 2.040 | 2.040 |
Rmerge | 0.150 | 0.440 |
Number of reflections | 810104 | |
<I/σ(I)> | 8.4 | 3.9 |
Completeness [%] | 98.5 | 91 |
Redundancy | 7.4 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 22 % (W/V) PEG (POLY[ETHYLENE GLYCOL]) MONOMETHYL ETHER 3350 AND 0.14 M MG(NO3)2, PH 7.5 |