4USS
Populus trichocarpa glutathione transferase X1-1 (GHR1), complexed with glutathione
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-10-08 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 36.530, 92.409, 86.901 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.525 - 2.500 |
R-factor | 0.1862 |
Rwork | 0.183 |
R-free | 0.23260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ppu |
RMSD bond length | 0.003 |
RMSD bond angle | 0.668 |
Data reduction software | XDS |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.530 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.160 | 0.790 |
Number of reflections | 10702 | |
<I/σ(I)> | 12.1 | 3 |
Completeness [%] | 99.6 | 82.1 |
Redundancy | 10.8 | 10.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |