4USR
Structure of flavin-containing monooxygenase from Pseudomonas stutzeri NF13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 120 |
| Collection date | 2014-03-12 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 63.560, 63.560, 189.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.940 - 1.830 |
| R-factor | 0.16438 |
| Rwork | 0.162 |
| R-free | 0.20433 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a9w |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.155 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.940 | 1.850 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.110 | 0.620 |
| Number of reflections | 41893 | |
| <I/σ(I)> | 14.9 | 3.8 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 9.8 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 35 % (W/V) TACSIMATE AT PH 7.0 |
