4USE
Human STK10 (LOK) with SB-633825
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-25 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.040, 50.250, 133.710 |
| Unit cell angles | 90.00, 101.16, 90.00 |
Refinement procedure
| Resolution | 131.180 - 2.650 |
| R-factor | 0.22598 |
| Rwork | 0.225 |
| R-free | 0.25145 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.268 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.590 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.120 | 0.910 |
| Number of reflections | 23790 | |
| <I/σ(I)> | 5.3 | 1.8 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 50% (V/V) PEG 300, 0.1M CACODYLATE PH 6.5, 0.2M MGCL2 |
