4USC
Crystal structure of peroxidase from palm tree Chamaerops excelsa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Collection date | 2011-05-13 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.180, 100.650, 132.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.110 - 2.600 |
| R-factor | 0.21964 |
| Rwork | 0.218 |
| R-free | 0.24585 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hdl |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.731 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.100 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.010 | 0.560 |
| Number of reflections | 29525 | |
| <I/σ(I)> | 10.4 | 3.7 |
| Completeness [%] | 90.1 | 91.8 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.17 M AMMONIUM SULFATE, 0.085 M TRIS PH 8.0, 17% PEG MME 2K, 15% GLYCEROL |
