4US4
Crystal Structure of the Bacterial NSS Member MhsT in an Occluded Inward-Facing State (lipidic cubic phase form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-25 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 44.200, 92.660, 112.500 |
| Unit cell angles | 90.00, 99.54, 90.00 |
Refinement procedure
| Resolution | 39.442 - 2.600 |
| R-factor | 0.205 |
| Rwork | 0.201 |
| R-free | 0.25450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4us3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.810 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX (AUTOMR) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.400 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.160 | 0.920 |
| Number of reflections | 13315 | |
| <I/σ(I)> | 5 | 1 |
| Completeness [%] | 96.8 | 97.6 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 7 | CRYSTALLISED BY LCP USING 7.8 MAG AS HOST LIPID. CRYSTALS WERE GROWN IN 20% PEG400, 400 MM NACL, 100 MM HEPES PH 7.0 |
