4US3
Crystal Structure of the bacterial NSS member MhsT in an Occluded Inward-Facing State
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 44.280, 49.890, 110.050 |
| Unit cell angles | 90.00, 96.76, 90.00 |
Refinement procedure
| Resolution | 43.972 - 2.098 |
| R-factor | 0.1921 |
| Rwork | 0.191 |
| R-free | 0.23420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PHENIX SCULPTOR MODEL WITH PDB ENTRY 2A65 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.239 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX (MR_ROSETTA) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.160 | 1.240 |
| Number of reflections | 27894 | |
| <I/σ(I)> | 8.9 | 1.15 |
| Completeness [%] | 98.7 | 98.5 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | CRYSTALLISED USING HILIDE WITH DOPC AS ADDED LIPID. CRYSTALS WERE OBTAINED IN 20-24% W/W PEG400, 400 MM NACL, 100 MM TRIS-HCL PH 7.0, 5% TRIMETHYLAMINE-N-OXIDE |
