4US1

The crystal structure of H-Ras and SOS in complex with ligands

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-15
Detector	ADSC CCD
Spacegroup name	I 4 2 2
Unit cell lengths	149.560, 149.560, 199.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.910 - 2.650
R-factor	0.193
Rwork	0.191
R-free	0.23130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bkd
RMSD bond length	0.010
RMSD bond angle	1.010
Data reduction software	MOSFLM
Refinement software	BUSTER (2.11.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.910	2.740
High resolution limit [Å]	2.650	2.650
Rmerge	0.120	0.860
Number of reflections	30489
<I/σ(I)>	13.7	2.3
Completeness [%]	92.9	94.3
Redundancy	7.2	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1