4US0

The crystal structure of H-Ras and SOS in complex with ligands

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Collection date	2010-04-08
Spacegroup name	I 4 2 2
Unit cell lengths	149.217, 149.217, 201.038
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	119.820 - 2.170
R-factor	0.187
Rwork	0.186
R-free	0.21230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bkd
RMSD bond length	0.010
RMSD bond angle	1.010
Data reduction software	XDS
Refinement software	BUSTER (2.11.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	119.820	2.290
High resolution limit [Å]	2.170	2.170
Rmerge	0.060	0.480
Number of reflections	59613
<I/σ(I)>	33.2	6.4
Completeness [%]	100.0	100
Redundancy	13.9	14.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1