4URU
The crystal structure of H-Ras and SOS in complex with ligands
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-08 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 149.282, 149.282, 200.517 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 105.560 - 2.830 |
| R-factor | 0.1921 |
| Rwork | 0.190 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.020 |
| Data reduction software | MOSFLM |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 119.740 | 2.980 |
| High resolution limit [Å] | 2.830 | 2.830 |
| Rmerge | 0.070 | 0.480 |
| Number of reflections | 27510 | |
| <I/σ(I)> | 30.7 | 5.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12 | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.2M SODIUM FORMATE, 2% DMSO |
