4URS
Crystal Structure of GGDEF domain from T.maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 291 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.150, 37.140, 62.280 |
| Unit cell angles | 95.48, 100.23, 107.78 |
Refinement procedure
| Resolution | 32.580 - 2.270 |
| R-factor | 0.2204 |
| Rwork | 0.218 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3icl |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.830 | 2.420 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.140 | 0.510 |
| Number of reflections | 13630 | |
| <I/σ(I)> | 6.5 | 2.6 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 4.6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M MONOBASIC SODIUM PHOSPHATE MONOHYDRATE, 0.1 M MONOBASIC POTASSIUM PHOSPHATE, 0.1 M MES MONOHYDRATE (PH 6.5), 2 M NACL |
