4URQ
Crystal Structure of GGDEF domain (I site mutant) from T.maritima
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 291 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 159.410, 91.980, 87.590 |
Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
Resolution | 38.370 - 2.500 |
R-factor | 0.2232 |
Rwork | 0.222 |
R-free | 0.24670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3icl |
RMSD bond length | 0.010 |
RMSD bond angle | 1.110 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | BALBES |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.970 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.090 | 0.690 |
Number of reflections | 43759 | |
<I/σ(I)> | 15.5 | 3 |
Completeness [%] | 99.7 | 100 |
Redundancy | 7.4 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 2 M NACL, 10% (W/V) PEG 6000 |