4URG
Crystal Structure of GGDEF domain from T.maritima (active-like dimer)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 291 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.890, 52.120, 73.710 |
| Unit cell angles | 90.00, 124.05, 90.00 |
Refinement procedure
| Resolution | 22.710 - 1.900 |
| R-factor | 0.2091 |
| Rwork | 0.207 |
| R-free | 0.24130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3icl |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.490 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.120 | 0.320 |
| Number of reflections | 27197 | |
| <I/σ(I)> | 11.4 | 5.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.8 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 0.15 M LITHIUM SULFATE, 0.1 M CITRIC ACID (PH 4.0), 10% (W/V) PEG 6000 |
