4UR6
Structure of the type III fish antifreeze protein from Zoarces viviparus ZvAFP6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-03 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.570, 108.170, 38.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.900 - 1.200 |
| R-factor | 0.17965 |
| Rwork | 0.179 |
| R-free | 0.19536 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ops |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.819 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.230 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.030 | 0.430 |
| Number of reflections | 300182 | |
| <I/σ(I)> | 25.2 | 5.2 |
| Completeness [%] | 98.5 | 94.9 |
| Redundancy | 6.2 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2.5 M (NH4)2SO4, 0.1 M CITRIC ACID, PH 4 |
