4UR4
Structure of the type III fish antifreeze protein from Zoarces viviparus ZvAFP13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-30 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.260, 39.690, 44.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.540 - 1.450 |
| R-factor | 0.13583 |
| Rwork | 0.134 |
| R-free | 0.17588 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4msi |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.864 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.540 | 1.490 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.040 | 0.310 |
| Number of reflections | 10321 | |
| <I/σ(I)> | 24.2 | 4.7 |
| Completeness [%] | 98.0 | 92.5 |
| Redundancy | 4.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2.5 M (NH4)2SO4, 0.1 M CITRIC ACID, PH 4.5 |
