4UQX
Coevolution of the ATPase ClpV, the TssB-TssC Sheath and the Accessory HsiE Protein Distinguishes Two Type VI Secretion Classes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-31 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.760, 45.870, 59.470 |
| Unit cell angles | 90.00, 109.98, 90.00 |
Refinement procedure
| Resolution | 34.780 - 1.200 |
| R-factor | 0.1512 |
| Rwork | 0.151 |
| R-free | 0.15770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zbp |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.280 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.900 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.060 | 0.530 |
| Number of reflections | 88118 | |
| <I/σ(I)> | 8.2 | 1.9 |
| Completeness [%] | 98.1 | 98.2 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 30% 2-METHYL 2,4-PENTANEDIOL 0.1 M SODIUM ACETATE (PH 4.6) 20 MM CACL2 |
