4UQE
X-ray structure of glucuronoxylan-xylanohydrolase (Xyn30A) from Clostridium thermocellum at 1.28 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-13 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.940, 46.070, 59.050 |
| Unit cell angles | 68.11, 80.19, 60.99 |
Refinement procedure
| Resolution | 40.180 - 1.280 |
| R-factor | 0.095 |
| Rwork | 0.094 |
| R-free | 0.11100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ckq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.504 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.180 | 1.300 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.060 | 0.200 |
| Number of reflections | 96062 | |
| <I/σ(I)> | 23 | 10.7 |
| Completeness [%] | 95.1 | 90.1 |
| Redundancy | 4.3 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25 % (V/V) PEG 3350, 0.1 M HEPES 7.5, 0.2 M CACL2 |
