4UQC
X-ray structure of glucuronoxylan-xylanohydrolase (Xyn30A) from Clostridium thermocellum at 1.30 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-13 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.390, 50.440, 58.190 |
| Unit cell angles | 111.90, 110.80, 97.67 |
Refinement procedure
| Resolution | 44.840 - 1.300 |
| R-factor | 0.113 |
| Rwork | 0.112 |
| R-free | 0.14100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ckq |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.562 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.840 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.060 | 0.180 |
| Number of reflections | 109732 | |
| <I/σ(I)> | 20.8 | 7.9 |
| Completeness [%] | 94.4 | 82.8 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.4 M K/NA TARTRATE |
