4UQB
X-ray structure of glucuronoxylan-xylanohydrolase (Xyn30A) from Clostridium thermocellum at 1.68 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-13 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.980, 50.590, 59.310 |
| Unit cell angles | 114.77, 101.43, 103.85 |
Refinement procedure
| Resolution | 45.490 - 1.680 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ckq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.212 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.680 | 1.740 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.080 | 0.830 |
| Number of reflections | 47651 | |
| <I/σ(I)> | 13.63 | 1.77 |
| Completeness [%] | 92.3 | 77.2 |
| Redundancy | 4.3 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES 7.5, 10 % (V/V)PEG 8000, 8 % (V/V)ETHILE GLYCOL |
