4UPF
Assembly principles of the unique cage formed by the ATPase RavA hexamer and the lysine decarboxylase LdcI decamer
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | FEI TECNAI F30 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
| Number of Images | 911 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 7.5 |
| Software | CTFFIND,Flex-EM,Situs,EMAN,FPM,IMAGIC,SPIDER |
| Symmetry | D5 |
