4UP9
Crystal structure of Entamoeba histolytica lysyl-tRNA synthetase in complex with ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-17 |
Detector | ADSC Q270 |
Spacegroup name | P 62 |
Unit cell lengths | 155.551, 155.551, 92.926 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.431 - 3.047 |
R-factor | 0.2186 |
Rwork | 0.216 |
R-free | 0.26250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bju |
RMSD bond length | 0.005 |
RMSD bond angle | 0.908 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.900 | 3.260 |
High resolution limit [Å] | 3.050 | 3.050 |
Rmerge | 0.200 | |
Number of reflections | 24433 | |
<I/σ(I)> | 8.8 | 1.1 |
Completeness [%] | 99.1 | 96.3 |
Redundancy | 6.2 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 50 MM HEPES PH 8.0, 50 MM NACL, 7.5% PEG 4000, 1.2 MM SPERMINE |