4UOQ

Nucleophile mutant (E324A) of beta-(1,6)-galactosidase from Bifidobacterium animalis subsp. lactis Bl-04

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-3
Synchrotron site	MAX II
Beamline	I911-3
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-10
Detector	MARRESEARCH MAR165
Spacegroup name	P 32 2 1
Unit cell lengths	150.560, 150.560, 237.250
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.930 - 2.700
R-factor	0.182
Rwork	0.181
R-free	0.22670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uni
RMSD bond length	0.009
RMSD bond angle	1.189
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.770
High resolution limit [Å]	2.700	2.700
Rmerge	0.090	0.600
Number of reflections	85508
<I/σ(I)>	24.65	4.4
Completeness [%]	99.5	100
Redundancy	11.7	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		29% PEG1500, 0.01 MM MGCL2, 0.1 M MMT BUFFER PH 8