4UOO
Structure of lipoteichoic acid synthase LtaS from Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-29 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 119.760, 119.760, 473.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 108.880 - 3.000 |
| R-factor | 0.22405 |
| Rwork | 0.222 |
| R-free | 0.25996 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w8d |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.790 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 106.880 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.120 | 0.560 |
| Number of reflections | 63877 | |
| <I/σ(I)> | 8.6 | 2.3 |
| Completeness [%] | 90.7 | 84.6 |
| Redundancy | 4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.64M NA ACETATE PH 4.6, 4% PEG3350, 100 MM MGCL2 |
