4UOJ
Structure of Fungal beta-mannosidase (GH2) from Trichoderma harzianum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Collection date | 2002-09-02 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 165.161, 165.631, 123.562 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 117.230 - 2.500 |
| R-factor | 0.17966 |
| Rwork | 0.179 |
| R-free | 0.20113 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.731 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.940 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.240 | 1.250 |
| Number of reflections | 117122 | |
| <I/σ(I)> | 4.34 | 2.1 |
| Completeness [%] | 99.6 | 96.8 |
| Redundancy | 7.8 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 26% PEG 400, 0.13 M CDCL2 AND 0.1 M SODIUM ACETATE PH 4.7 |
