4UOC
Competence or damage-inducible protein CinA from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.470, 93.300, 133.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 93.000 - 2.460 |
| R-factor | 0.19202 |
| Rwork | 0.189 |
| R-free | 0.25041 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ct8 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.792 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.000 | 2.520 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.120 | 0.700 |
| Number of reflections | 31049 | |
| <I/σ(I)> | 7.9 | 2 |
| Completeness [%] | 93.4 | 95.9 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M SODIUM SULFATE 0.1 M BIS-TRIS PROPANE 20 % (W/V) PEG 3350 |
