4UOB
Crystal structure of Deinococcus radiodurans Endonuclease III-3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Wavelength(s) | 1.07812, 1.735 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 91.240, 40.510, 72.150 |
Unit cell angles | 90.00, 102.34, 90.00 |
Refinement procedure
Resolution | 41.932 - 1.310 |
R-factor | 0.1348 |
Rwork | 0.134 |
R-free | 0.15930 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.010 |
RMSD bond angle | 1.257 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | Auto-Rickshaw |
Refinement software | PHENIX ((PHENIX.REFINE: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.340 |
High resolution limit [Å] | 1.310 | 1.310 |
Rmerge | 0.060 | 0.300 |
Number of reflections | 61009 | |
<I/σ(I)> | 17.4 | 2.1 |
Completeness [%] | 98.2 | 90.2 |
Redundancy | 6.8 | 1.87 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 0.1M MES PH6.5, 1.4M AMMONIUM SULPHATE AND 0.01M COBALT (II) CHLORIDE HEXAHYDRATE |