4UO9
Structure of the A_Canine_Colorado_17864_06 H3 haemagglutinin Ser30Thr mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 100.382, 100.382, 396.534 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 132.180 - 3.200 |
| R-factor | 0.22095 |
| Rwork | 0.218 |
| R-free | 0.27374 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uo4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.151 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.970 | 3.420 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.100 | 0.600 |
| Number of reflections | 12600 | |
| <I/σ(I)> | 12.2 | 2.5 |
| Completeness [%] | 96.8 | 96.8 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
