4UO3
Structure of the A_Equine_Richmond_07 H3 haemagglutinin mutant Ser30Thr
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.820, 129.620, 195.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.890 - 2.870 |
R-factor | 0.22038 |
Rwork | 0.217 |
R-free | 0.27664 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4uo0 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.313 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 129.620 | 2.940 |
High resolution limit [Å] | 2.870 | 2.870 |
Rmerge | 0.130 | 0.740 |
Number of reflections | 46666 | |
<I/σ(I)> | 11.3 | 2.6 |
Completeness [%] | 99.0 | 100 |
Redundancy | 6.3 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |