4UO2

Structure of the A_Equine_Richmond_07 H3 haemagglutinin in complex with Sialyl Lewis X

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	79.296, 129.794, 193.634
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	107.810 - 2.700
R-factor	0.20065
Rwork	0.198
R-free	0.25591
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uo0
RMSD bond length	0.006
RMSD bond angle	1.134
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.460	2.790
High resolution limit [Å]	2.700	2.700
Rmerge	0.130	0.710
Number of reflections	55175
<I/σ(I)>	8	2.38
Completeness [%]	99.1	99.9
Redundancy	4.4	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1