4UNM
Structure of Galactose Oxidase homologue from Streptomyces lividans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-23 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.360, 126.630, 107.550 |
Unit cell angles | 90.00, 91.10, 90.00 |
Refinement procedure
Resolution | 53.500 - 1.770 |
R-factor | 0.18971 |
Rwork | 0.188 |
R-free | 0.22819 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2EIE (MODIFIED WITH CHAINSAW) |
RMSD bond length | 0.011 |
RMSD bond angle | 1.414 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.300 | 1.800 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.060 | 0.630 |
Number of reflections | 130533 | |
<I/σ(I)> | 10.5 | 2 |
Completeness [%] | 99.7 | 99.6 |
Redundancy | 3.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4 | 0.1M NA ACETATE PH 4, 20% PEG 20000 |