4UNI
beta-(1,6)-galactosidase from Bifidobacterium animalis subsp. lactis Bl-04 in complex with galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-28 |
| Detector | MARRESEARCH MAR165 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 139.520, 199.440, 217.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.727 - 2.600 |
| R-factor | 0.1766 |
| Rwork | 0.175 |
| R-free | 0.23410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kwg |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.614 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.100 | 0.910 |
| Number of reflections | 92722 | |
| <I/σ(I)> | 19.07 | 2.6 |
| Completeness [%] | 99.5 | 94.8 |
| Redundancy | 8.2 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 27% PEG1500, 0.01 M MGCL2, 0.1 M MMT BUFFER PH 8 |
