4UNG
Human insulin B26Asn mutant crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-04 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 45.630, 45.630, 117.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.060 - 1.810 |
| R-factor | 0.17594 |
| Rwork | 0.174 |
| R-free | 0.21992 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mso |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.782 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0033) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.630 | 1.850 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.110 | 0.820 |
| Number of reflections | 12092 | |
| <I/σ(I)> | 19.1 | 4.2 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 14.2 | 15.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 0.035 M (NH4)2SO4, PH 4.0, CP = 5 MG/ML |
