4UN2
Crystal structure of the UBA domain of Dsk2 in complex with Ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-05-27 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 77.971, 77.972, 49.485 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 67.520 - 1.510 |
| R-factor | 0.15921 |
| Rwork | 0.157 |
| R-free | 0.19615 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1UBQ AND 2BWB |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.087 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.500 | 1.620 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.050 | 0.370 |
| Number of reflections | 27296 | |
| <I/σ(I)> | 27.78 | 4.55 |
| Completeness [%] | 99.5 | 98.1 |
| Redundancy | 8.07 | 7.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.2M MAGNESIUM CHLORIDE, 0.1M TRIS PH 8.5, 20% PEG 8000 |
