4UN2
Crystal structure of the UBA domain of Dsk2 in complex with Ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-05-27 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 77.971, 77.972, 49.485 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 67.520 - 1.510 |
R-factor | 0.15921 |
Rwork | 0.157 |
R-free | 0.19615 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1UBQ AND 2BWB |
RMSD bond length | 0.023 |
RMSD bond angle | 2.087 |
Data reduction software | XDS |
Data scaling software | SADABS |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.500 | 1.620 |
High resolution limit [Å] | 1.520 | 1.520 |
Rmerge | 0.050 | 0.370 |
Number of reflections | 27296 | |
<I/σ(I)> | 27.78 | 4.55 |
Completeness [%] | 99.5 | 98.1 |
Redundancy | 8.07 | 7.93 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 0.2M MAGNESIUM CHLORIDE, 0.1M TRIS PH 8.5, 20% PEG 8000 |