4UN1
Sirohaem decarboxylase AhbA/B - an enzyme with structural homology to the Lrp/AsnC transcription factor family that is part of the alternative haem biosynthesis pathway.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Collection date | 2014-04-08 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.300, 78.600, 150.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.230 - 1.970 |
| R-factor | 0.2049 |
| Rwork | 0.202 |
| R-free | 0.25732 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4czd |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.235 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 2.020 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.080 | 0.800 |
| Number of reflections | 46648 | |
| <I/σ(I)> | 13.38 | 2.5 |
| Completeness [%] | 99.2 | 97 |
| Redundancy | 5.3 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 0.1M SODIUM ACETATE PH5, 0.2M AMMONIUM ACETATE, 30% PEG 4000, 0.01M BACL2, pH 5.0 |
