4UMW
CRYSTAL STRUCTURE OF A ZINC-TRANSPORTING PIB-TYPE ATPASE IN E2.PI STATE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-24 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 77.641, 83.560, 319.818 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.347 - 2.705 |
R-factor | 0.2251 |
Rwork | 0.224 |
R-free | 0.24060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rfu |
RMSD bond length | 0.005 |
RMSD bond angle | 0.932 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.870 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.060 | 1.120 |
Number of reflections | 28953 | |
<I/σ(I)> | 15.47 | 1.11 |
Completeness [%] | 99.7 | 99.1 |
Redundancy | 4.6 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 300 MM LITHIUM ACETATE, 3% V/V T-BUOH, 14 % POLY ETHYLENE GLYCOL 2000 MONOMETHYL ETHER (PEG2000-MME), 7 % V/V SORBITOL,10 % V/V GLYCEROL AND 5 MM BME |