4UMG
Crystal structure of the Lin-41 filamin domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 44.780, 52.110, 101.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.450 - 1.680 |
| R-factor | 0.2199 |
| Rwork | 0.219 |
| R-free | 0.24370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.720 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.040 | 0.880 |
| Number of reflections | 13883 | |
| <I/σ(I)> | 20.8 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.1 M SODIUM MALONATE, 0.1 M HEPES PH 7.0, 0.5% JEFFAMINE ED-2001 |
